2,592 research outputs found

    Shaping 21st Century Journalism: Leveraging a "Teaching Hospital Model" in Journalism Education

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    Calls on journalism programs to become "anchor institutions" in the digitally networked age by pursuing a broader, community-oriented mission, testing new journalism models, exploring how journalistic ecosystems evolve, and shaping policymaking processes

    Cytogenomic Analyses of the genus Sorghum

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    A phylogenetic tree based on ITS1, Adh1 and ndhF grouped the species of the genus Sorghum into one distinct monophyletic group, but including two sister lineages, one with x=5, the other with x=10 as basic chromosome numbers. The goal of this study was to elucidate major patterns in Sorghum genome evolution, particularly n=5 vs. n=10 genomes. A very recent molecular cytogenetic study in our laboratory revealed striking structural karyotypic rearrangements between S. bicolor (x=10) and an x=5 Sorghum species, S. angustum; so an immediate objective here was to determine if identical or similar rearrangements exist in other wild Sorghum species. Our approach was [1] to extend similar methods to additional species, i.e., fluorescent in situ hybridization (FISH) analyses of sorghum genomic bacterial artificial chromosome clones and multi-BAC cocktail probes to mitotic chromosomes of S. angustum, S. versicolor, S. brachypodum and S. intrans; and [2] to augment the BAC-FISH findings by comparing telomeric and ribosomal DNA FISH signal distributions to x=5 and x=10 Sorghum species. Signals from in situ hybridizations of BAC-based probes were insufficiently robust and insufficiently localized to delineate FISH signal patterns akin to those discovered previously in S. angustum. Southern blots of the same BACs to restricted DNA of these species revealed relatively moderate affinity to smeared DNA, suggesting homology to non-tandemized sequences. FISH of the A-type TRS (Arabidopsis-like telomeric repeat sequence) revealed its presence is limited to terminal chromosomal regions of the Sorghum species tested, except S. brachypodum, which displayed intercalary signal on one chromosome and no detachable signal at its termini region. The hybridization of 45S and 5S rDNA revealed that the respective sites of tandemized clusters differ among species in terms of size, number and location, except S. angustum versus S. versicolor. Well localized BAC-FISH signals normally occur when signals from low-copy sequences discernibly exceed background signal, including those from hybridization of dispersed repetitive elements. The low level of signal intensity from BAC low-copy sequences relative to the background signal "noise" seems most likely due to low homology and(or) technical constraints. Extensive dispersal of low-copy sequences that are syntenic in S. bicolor seems unlikely, but possible. In conclusion, the result was a lack of clear experimental success with BAC-FISH and an inability to effectively screen for S. angustum-like rearrangements using BAC-FISH. The telomeric and rDNA FISH indicated that the x=5 genomes vary extensively. One can surmise that although the arrangements seen in S. angustum might extend to S. versicolor, they certainly do not extend to S. versicolor, they certainly do not extend to S. intrans or S. brachypodum. It is clear that S. brachypodum has telomeric repeats that are either very short or rely on some sequence other than the A-type TRS

    Speed Management and Speed Reduction in Portland, OR

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    In 2015, the Portland City Council unanimously passed a resolution committing Portland to Vision Zero, the goal to eliminate traffic deaths and serious injuries. An underpinning of Vision Zero is that streets are managed for safe speeds. This presentation will summarize Portland\u27s speed management process, how it relates to achieving Vision Zero, and present two case studies in which speed limits were reduced: (1) a 25 mi/h to 20 mi/h reduction on residential streets and (2) various reductions on arterials and collectors. Reduction sites in which additional treatments were implemented, such as speed humps and fixed speed safety cameras, will also be discussed. Results of the data analysis will be shared, along with next steps in Portland\u27s speed management process.https://pdxscholar.library.pdx.edu/trec_seminar/1229/thumbnail.jp

    Understanding Factors Affecting Arterial Reliability Performance Metrics

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    In recent years, the importance of travel time reliability has become equally important as average travel time. However, the majority focus of travel time research is average travel time or travel time reliability on freeways. In addition, the identification of specific factors (i.e., peak hours, nighttime hours, etc.) and their effects on average travel time and travel time variability are often unknown. The current study addresses these two issues through a travel time-based study on urban arterials. Using travel times collected via Bluetooth data, a series of analyses are conducted to understand factors affecting reliability metrics on urban arterials. Analyses include outlier detection, a detailed descriptive analysis of select corridors, median travel time analysis, assessment of travel time reliability metrics recommended by the Federal Highway Administration (FHWA), and a bivariate Tobit model. Results show that day of the week, time of day, and holidays have varying effects on average travel time, travel time reliability, and travel time variability. Results also show that evening peak hours have the greatest effects in regards to increasing travel time, nighttime hours have the greatest effects in regards to decreasing travel time, and directionality plays a vital role in all travel time-related metrics

    A Temporal Investigation of Crash Severity Factors in Worker-Involved Work Zone Crashes: Random Parameters and Machine Learning Approaches

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    In the context of work zone safety, worker presence and its impact on crash severity has been less explored. Moreover, there is a lack of research on contributing factors by time-of-day. To accomplish this, first a mixed logit model was used to determine statistically significant crash severity contributing factors and their effects. Significant factors in both models included work-zone-specific characteristics and crash-specific characteristics, where environmental characteristics were only significant in the daytime model. In addition, results from parameter transferability test provided evidence that daytime and nighttime crashes need to be modeled separately. Further, to explore the nonlinear relationship between crash severity levels and time-of-day, as well as compare the effects of variables to that of the logit model and assess prediction performance, a Support Vector Machines (SVM) model trained by Cuckoo Search (CS) algorithm was utilized. Opening the SVM black-box, a variable impact analysis was also performed. In addition to the characteristics identified in the logit models, the SVM models also included the impacts of vehicle-level characteristics. The variable impact analysis illustrated that the termination area of the work zone is most critical for both daytime and nighttime crashes, as this location has the highest increase in severe injury likelihood. In summary, results of this study demonstrate that work zone crashes need to be modeled separately by time-of-day with a high level of confidence. Furthermore, results show that the CS-SVM models provide better prediction performance compared to the SVM and logit models

    Using Temporal Changes in Drought Indices to Generate Probabilistic Drought Intensification Forecasts

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    In this study, the potential utility of using rapid temporal changes in drought indices to provide early warning of an elevated risk for drought development over subseasonal time scales is assessed. Standardized change anomalies were computed each week during the 2000–13 growing seasons for drought indices depicting anomalies in evapotranspiration, precipitation, and soil moisture. A rapid change index (RCI) that encapsulates the accumulated magnitude of rapid changes in the weekly anomalies was computed each week for each drought index, and then a simple statistical method was used to convert the RCI values into drought intensification probabilities depicting the likelihood that drought severity as analyzed by the U.S. Drought Monitor (USDM) would worsen in subsequent weeks. Local and regional case study analyses revealed that elevated drought intensification probabilities often occur several weeks prior to changes in the USDM and in topsoil moisture and crop condition datasets compiled by the National Agricultural Statistics Service. Statistical analyses showed that the RCI-derived probabilities are most reliable and skillful over the central and eastern United States in regions most susceptible to rapid drought development. Taken together, these results suggest that tools used to identify areas experiencing rapid changes in drought indices may be useful components of future drought early warning systems

    Cytogenomic Analyses of the genus Sorghum

    Get PDF
    A phylogenetic tree based on ITS1, Adh1 and ndhF grouped the species of the genus Sorghum into one distinct monophyletic group, but including two sister lineages, one with x=5, the other with x=10 as basic chromosome numbers. The goal of this study was to elucidate major patterns in Sorghum genome evolution, particularly n=5 vs. n=10 genomes. A very recent molecular cytogenetic study in our laboratory revealed striking structural karyotypic rearrangements between S. bicolor (x=10) and an x=5 Sorghum species, S. angustum; so an immediate objective here was to determine if identical or similar rearrangements exist in other wild Sorghum species. Our approach was [1] to extend similar methods to additional species, i.e., fluorescent in situ hybridization (FISH) analyses of sorghum genomic bacterial artificial chromosome clones and multi-BAC cocktail probes to mitotic chromosomes of S. angustum, S. versicolor, S. brachypodum and S. intrans; and [2] to augment the BAC-FISH findings by comparing telomeric and ribosomal DNA FISH signal distributions to x=5 and x=10 Sorghum species. Signals from in situ hybridizations of BAC-based probes were insufficiently robust and insufficiently localized to delineate FISH signal patterns akin to those discovered previously in S. angustum. Southern blots of the same BACs to restricted DNA of these species revealed relatively moderate affinity to smeared DNA, suggesting homology to non-tandemized sequences. FISH of the A-type TRS (Arabidopsis-like telomeric repeat sequence) revealed its presence is limited to terminal chromosomal regions of the Sorghum species tested, except S. brachypodum, which displayed intercalary signal on one chromosome and no detachable signal at its termini region. The hybridization of 45S and 5S rDNA revealed that the respective sites of tandemized clusters differ among species in terms of size, number and location, except S. angustum versus S. versicolor. Well localized BAC-FISH signals normally occur when signals from low-copy sequences discernibly exceed background signal, including those from hybridization of dispersed repetitive elements. The low level of signal intensity from BAC low-copy sequences relative to the background signal "noise" seems most likely due to low homology and(or) technical constraints. Extensive dispersal of low-copy sequences that are syntenic in S. bicolor seems unlikely, but possible. In conclusion, the result was a lack of clear experimental success with BAC-FISH and an inability to effectively screen for S. angustum-like rearrangements using BAC-FISH. The telomeric and rDNA FISH indicated that the x=5 genomes vary extensively. One can surmise that although the arrangements seen in S. angustum might extend to S. versicolor, they certainly do not extend to S. versicolor, they certainly do not extend to S. intrans or S. brachypodum. It is clear that S. brachypodum has telomeric repeats that are either very short or rely on some sequence other than the A-type TRS

    Soil-less Agriculture at the Wat Pathumwanaram School: Expansion through an Aeroponic Garden and Educational Material

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    Working in a multinational team with students from a Thai university, we created an aeroponic garden for the Wat Pathumwanaram School\u27s soil-less agriculture program. Further, we created educational material to teach the practices of soil-less agriculture. These two objectives help the school demonstrate the role of soil-less agriculture as a sustainable technology. To develop the aeroponic garden we used engineering design, guiding us to a feasible solution to meet the needs of the school. In developing the curricular materials, we defined goals and outcomes that were matched to practices already used. This ensured that the changes could be seamlessly integrated into daily practice. Overall, we were able to help demonstrate the potential that technology has to meet the needs of a society

    Knowledge-based vision and simple visual machines

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    The vast majority of work in machine vision emphasizes the representation of perceived objects and events: it is these internal representations that incorporate the 'knowledge' in knowledge-based vision or form the 'models' in model-based vision. In this paper, we discuss simple machine vision systems developed by artificial evolution rather than traditional engineering design techniques, and note that the task of identifying internal representations within such systems is made difficult by the lack of an operational definition of representation at the causal mechanistic level. Consequently, we question the nature and indeed the existence of representations posited to be used within natural vision systems (i.e. animals). We conclude that representations argued for on a priori grounds by external observers of a particular vision system may well be illusory, and are at best place-holders for yet-to-be-identified causal mechanistic interactions. That is, applying the knowledge-based vision approach in the understanding of evolved systems (machines or animals) may well lead to theories and models that are internally consistent, computationally plausible, and entirely wrong
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